Biochem/physiol Actions

Cell permeable: no

Reversible: no

kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM

Product does not compete with ATP.

Primary TargetFluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity

General description

An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (k_cat = 21.6 s^-1 when the substrate concentration is half the V_max, S_0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.

An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (k_cat = 21.6 s^-1 and substrate concentration at half the V_max, S_0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jin, G., et al. 2002. Anal. Biochem.302, 269.

Packaging

1 mg in Glass bottle

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sequence

Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa

Warning

Toxicity: Standard Handling (A)